ELISA stands for Enzyme-Linked Immunosorbent Assay
Elisa Testing is meant to help us find out weather you are sick of something or contaiged of a virus. It is also considered a diagnostic tool and as a quality control check tool, Elisa testing is based in color change if there is a color changed then what you are solving for is infected or contains virus. For espample if you are solving for weather your Elisa lab is about small pox and you want to know if you are contaiged then you do the procedure and if at the en there is a color change in your results then you are contaiged of small pox. Also in the procedure there is a specific way of making it it is called ''sandwich Elisa'' and is formed of the following steps:
- Prepare a surface to which a known quantity of capture antibody is bound.
- Block any nonspecific binding sites on the surface.
- Apply the antigen-containing sample to the plate.
- Wash the plate, so that unbound antigen is removed.
- Apply enzyme linked primary antibodies as detection antibodies that also bind specifically to the antigen.
- Wash the plate, so that the unbound antibody-enzyme conjugates are removed.
- Apply a chemical that is converted by the enzyme into a color or fluorescent or electrochemical signal.
- Measure the absorbency or fluorescence or electrochemical signal of the plate wells to determine the presence and quantity of antigen.
In my experience with the Elisa test our setting was to find out weather if we were going to be contaiged with small pox and followed all these steps and our substance was contaiged of small pox. I determined that because at the end of the test there was a color change.
